Associate Research Grade Production
etherna immunotherapies📍 NielFunctiebeschrijving
The Associate Research Grade Production’s duties shall include, but not be limited to:
- Primary responsibility will be to produce research grade mRNA-LNPs, including lipid stock preparation, mRNA encapsulation (e.g., microfluidic mixing), buffer exchange/purification, concentration and sterile filtration.
- Perform in-process and release characterization of LNP formulations (e.g., particle size and polydispersity by DLS, zeta potential, encapsulation efficiency and mRNA content by RiboGreen assay, pH and osmolality).
- Prepare, aliquot and store LNP batches under appropriate conditions and follow up on their stability.
- Execute new techniques/assays and support the optimization and scale-up of formulation processes.Work collaboratively with other departments (mRNA production, QC, Process Development) and/or project managers.
- Maintain appropriate files related to ongoing formulations. Write formulation protocols, batch records, analytical test methods and reports if necessary.
- Keep track of QC results of ongoing productions.
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